Antibiotic Resistance Markers for Genetic Manipulations of Leptospira spp-Reference-Cited by-同舟云学术

Antibiotic Resistance Markers for Genetic Manipulations of Leptospira spp

Published:2010-07-15 Issue:14 Volume:76 Page:4882-4885
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Poggi Dante¹,Oliveira de Giuseppe Priscila¹,Picardeau Mathieu¹

Affiliation:

1. Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France

Abstract

ABSTRACT We measured the frequency of appearance of spontaneous mutants resistant to gentamicin, kanamycin, streptomycin, and spectinomycin in saprophytic and pathogenic Leptospira strains. The mutations responsible for the spontaneous resistance to streptomycin and spectinomycin were identified in the rpsL and rrs genes, respectively. We also generated a gentamicin resistance cassette that allows the use of a third selectable marker in leptospires. These results may facilitate further advances in gene transfer systems in Leptospira spp.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00775-10

Reference22 articles.

1. Bauby, H., I. Saint Girons, and M. Picardeau. 2003. Construction and complementation of the first auxotrophic mutant in the spirochaete Leptospira meyeri. Microbiology149:689-693.

2. Random Insertional Mutagenesis of Leptospira interrogans , the Agent of Leptospirosis, Using a mariner Transposon

3. Mutations Conferring Aminoglycoside and Spectinomycin Resistance in Borrelia burgdorferi

4. Clonal Polymorphism of Borrelia burgdorferi Strain B31 MI: Implications for Mutagenesis in an Infectious Strain Background

5. Fromm, H., M. Edelman, D. Aviv, and E. Galun. 1987. The molecular basis for rDNA-dependent spectinomycin resistance in Nicotiana chloroplasts. EMBO J.6:3233-3237.

Cited by 25 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Encapsulated Phage Cocktail: The Destined Environmental Biocontrol Agent For Pathogenic Leptospira;Malaysian Applied Biology;2024-06-30

2. Exploring Leptospira interrogans FDAARGOS_203: Insights into AMR and Anti-Phage Defense;Microorganisms;2024-03-08

3. Structural investigation of vitamin K epoxide reductase domain-containing protein in Leptospira species: a potential target for the development of new leptospirosis treatments as an alternative to antibiotics;Journal of Biomolecular Structure and Dynamics;2024-01-10

4. Nanotherapeutics for Bacterial Livestock Diseases;Livestock Diseases and Management;2024

5. Evaluation of Leptospira interrogans knockdown mutants for LipL32, LipL41, LipL21, and OmpL1 proteins;Frontiers in Microbiology;2023-06-23