Properties of Purified Pneumococcal Hemolysin-Reference-Cited by-同舟云学术

Properties of Purified Pneumococcal Hemolysin

Published:1972-11 Issue:5 Volume:6 Page:755-760
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Johnson Mary K.¹

Affiliation:

1. Departments of Ophthalmology and Microbiology, Tulane University School of Medicine, New Orleans, Louisiana 70112

Abstract

With a purified preparation of pneumococcal hemolysin, a sigmoid relationship was found to exist between lysin concentration and hemolytic activity. Hemolysis was inhibited by a high ratio of erythrocytes to lysin, suggesting a multi-hit mechanism of action. Reaction rate decreased rapidly with time, possibly due to competition between ghosts and unlysed erythrocytes for fixation of lysin. The effects of p H and various agents on the processes of lysin adsorption and hemoglobin release were determined. The pneumococcal preparation did not possess nicotinamide adenine dinucleotide glycohydrolase activity.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.6.5.755-760.1972

Reference9 articles.

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3. ThW preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes;Dodge J. T.;Arch. Biochem.,1963

4. Reinigung und Kristallisation der NAD-Glykohydrolase aus C-Streptokokken;Fehrenbach F. J.;Eur. J. Biochem.,1971

5. Identity of streptolysin-O and NADglycohydrolase (BC 3.2.2.5);Fehrenbach F. J.;Z. Naturforsch. Ser. B,1971

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