Affiliation:
1. Departments of Physiology, Anesthesiology, and Microbiology, Jefferson Medical College, Philadelphia, Pennsylvania 19107
Abstract
The in vitro effects of high O
2
tensions (P
O
2
) on aerobic, enteric pathogens were examined at pressures of up to 3 atm absolute. Organisms from the genera
Salmonella, Shigella
, and
Vibrio
were usually subjected to 24-hr exposures. Tensions of 0.87, 1.87, and 2.87 atm absolute of O
2
(plus traces of CO
2
and N
2
) became progressively inhibitory for
Salmonella
and
Shigella
growth, but were bactericidal only for
V. comma
strains at tensions greater than 0.87 atm absolute of O
2
. Growth inhibition of enteric organisms resulted from increased P
O
2
, rather than pressure per se, and could be mitigated nutritionally; an appropriate carbohydrate source is at least partially involved. Further studies with vibrios indicated that such mitigation was independent of medium
p
H. In addition, a synergistic relationship existed between O
2
and sulfisoxazole when tensions from 0.87 to 2.87 atm absolute of O
2
were maintained for 3 to 24 hr. Synergism occurred even under nutritional conditions which negated growth inhibition by O
2
alone. Bactericidal concentrations of sulfisoxazole, in the presence of increased P
O
2
, were reducible up to 4,000-fold. The combined procedure employed in this investigation, by use of an antimicrobial drug of known action, which also synergizes with O
2
, plus nutritional studies, suggests a means for establishing a site of O
2
toxicity. These data support the concept that O
2
inhibition of growth represents a metabolic disturbance and that metabolic pathways involving
p
-aminobenzoic acid may be O
2
-labile. Such an approach could also guide development of antimicrobial agents as O
2
substitutes for promoting synergism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference12 articles.
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