Affiliation:
1. MedImmune Vaccines, Inc., Mountain View, California 94043
Abstract
ABSTRACT
The four temperature-sensitive (
ts
) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus with these four introduced
ts
loci exhibited both
ts
and
att
phenotypes similar to those of MDV-A, which could be used as a donor virus for manufacturing large quantities of inactivated influenza virus vaccine against potential pandemic strains.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference19 articles.
1. Anchlan, D., S. Ludwig, P. Nymadawa, J. Mendsaikhan, and C. Scholtissek. 1996. Previous H1N1 influenza A viruses circulating in the Mongolian population. Arch. Virol.141:1553-1569.
2. Beare, A. S., G. C. Schild, and J. W. Craig. 1975. Trials in man with live recombinants made from A/PR/8/34 (H0 N1) and wild H3 N2 influenza viruses. Lancetii:729-732.
3. Ennis, F. A., M. Verbonitz, P. Reichelderfer, and S. Daniel. 1976. Recombination of influenza A virus strains: effect on pathogenicity. Dev. Biol. Stand.33:220-225.
4. A Single Amino Acid Mutation in the PA Subunit of the Influenza Virus RNA Polymerase Inhibits Endonucleolytic Cleavage of Capped RNAs
5. Rescue of Influenza A Virus from Recombinant DNA