Rescue of Influenza A Virus from Recombinant DNA-Reference-Cited by-同舟云学术

Rescue of Influenza A Virus from Recombinant DNA

Published:1999-11 Issue:11 Volume:73 Page:9679-9682
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Fodor Ervin¹²,Devenish Louise¹,Engelhardt Othmar G.²,Palese Peter²,Brownlee George G.¹,García-Sastre Adolfo²

Affiliation:

1. Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom,1 and

2. Department of Microbiology, Mount Sinai School of Medicine, New York, New York 100292

Abstract

ABSTRACT We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.73.11.9679-9682.1999

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