Ultrasensitive Detection of Clostridioides difficile Toxins in Stool by Use of Single-Molecule Counting Technology: Comparison with Detection of Free Toxin by Cell Culture Cytotoxicity Neutralization Assay

Author:

Hansen Glen12,Young Stephen34,Wu Alan H. B.5,Herding Emily12,Nordberg Vickie12,Mills Ray3,Griego-Fullbright Christen3,Wagner Aaron3,Ong Chui Mei5,Lewis Shawna6,Yoon Joseph6,Estis Joel7,Sandlund Johanna7,Friedland Emily7,Carroll Karen C.6

Affiliation:

1. Department of Pathology, Hennepin County Medical Center, Minneapolis, Minnesota, USA

2. Division of Infectious Diseases, Department of Pathology and Laboratory Medicine, University of Minnesota, Minneapolis, Minnesota, USA

3. TriCore Reference Laboratories, Albuquerque, New Mexico, USA

4. Department of Pathology, University of New Mexico HSC, Albuquerque, New Mexico, USA

5. Department of Laboratory Medicine, University of California, Zuckerberg San Francisco General Hospital, San Francisco, California, USA

6. Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

7. Singulex, Inc., Alameda, California, USA

Abstract

Laboratory tests for Clostridioides difficile infection (CDI) rely on the detection of free toxin or molecular detection of toxin genes. The Singulex Clarity C. diff toxins A/B assay is a rapid, automated, and ultrasensitive assay that detects C. difficile toxins A and B in stool. We compared CDI assays across two prospective multicenter studies to set a cutoff for the Clarity assay and to independently validate the performance compared with that of a cell culture cytotoxicity neutralization assay (CCCNA).

Funder

Singulex, Inc.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3