bla VIM-2 and bla VIM-7 Carbapenemase-Producing Pseudomonas aeruginosa Isolates Detected in a Tertiary Care Medical Center in the United States: Report from the MYSTIC Program

Abstract

ABSTRACT Two Pseudomonas aeruginosa strains resistant to beta-lactams, fluoroquinolones, aminoglycosides, tetracyclines, and carbapenems and susceptible only to polymyxin B (MIC ≤ 2 μg/ml) were identified as part of the Meropenem Yearly Susceptibility Test Information Collection program. Metallo-β-lactamase screening tests were positive, PCR yielded products with bla VIM primers, and sequence analysis revealed bla VIM-7 and bla VIM-2 . The isolates had distinct ribotype and pulsed-field gel electorphoresis patterns and appeared independently, remote in time and location, at the same cancer center.