Multiplex PCR Assay Targeting a Diguanylate Cyclase-Encoding Gene, cgcA , To Differentiate Species within the Genus Cronobacter-Reference-Cited by-同舟云学术

Multiplex PCR Assay Targeting a Diguanylate Cyclase-Encoding Gene, cgcA , To Differentiate Species within the Genus Cronobacter

Published:2013-01-15 Issue:2 Volume:79 Page:734-737
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Carter L.¹,Lindsey L. A.¹,Grim C. J.¹²,Sathyamoorthy V.¹,Jarvis K. G.¹,Gopinath G.¹,Lee C.¹,Sadowski J. A.¹,Trach L.¹,Pava-Ripoll M.³,McCardell B. A.¹,Tall B. D.¹,Hu L.¹²

Affiliation:

1. CFSAN, FDA, Laurel, Maryland, USA

2. Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA

3. CFSAN, FDA, College Park, Maryland, USA

Abstract

ABSTRACT In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA , a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02898-12

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