Affiliation:
1. Department of Biotechnology and Genetic Engineering Jordan University of Science and Technology Irbid Jordan
2. Department of Nutrition and Food Technology Jordan University of Science and Technology Irbid Jordan
3. US Food and Drug Administration Center for Food Safety and Applied Nutrition Laurel Maryland USA
Abstract
Abstract
Aims
The aim was to characterize a collection of Cronobacter sakazakii isolates collected from various origins in Jordan.
Methods and Results
The isolates were characterized using 16S rRNA sequencing, DNA microarray, multi-locus sequence typing (MLST), O-serotyping, virulence gene identification and antibiotic susceptibility testing. The identities and phylogenetic relatedness revealed that C. sakazakii sequence type 4 (ST4) and Csak O:1 serotype were the most prevalent STs and serovars amongst these C. sakazakii strains. PCR screening of putative virulence genes showed that the siderophore-interacting protein gene (sip) and iron acquisition gene clusters (eitCBAD and iucABCD/iutA) were the most detected genes with noticeable variability in the type 6 secretion system (T6SS) and filamentous hemagglutinin/adhesion (FHA) gene loci. The antibiotic resistance profiles revealed that the majority of the isolates were susceptible to all antibiotics used despite harbouring a class C β-lactamase resistance gene.
Conclusions
The results described in this report provide additional insights about the considerable genotypic and phenotypic heterogeneity within C. sakazakii.
Significance and Impact of the Study
The information reported in this study might be of great value in understanding the origins of C. sakazakii isolates, in addition to their diversity and variability, which might be helpful in preventing future outbreaks of this pathogen.
Funder
Deanship of Research, Jordan University of Science and Technology
US FDA
Jordan University of Science and Technology
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Cited by
3 articles.
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