Affiliation:
1. Unité des Interactions Bactéries-Cellules, Institut Pasteur, 75724 Paris Cedex 15, France
Abstract
ABSTRACT
To investigate factors which modulate the entry of
Listeria monocytogenes
into mammalian cells, we have analyzed the role of Ca
2+
. We show that
L. monocytogenes
induced Ca
2+
transients into the human Hep-2 epithelial cell line. The nonpathogenic species
L. innocua
or a
L. monocytogenes
mutant strain defective in listeriolysin O (LLO) production was unable to induce these calcium fluxes. Addition of plasma membrane calcium channel antagonists or chelation of extracellular calcium markedly reduced
L. monocytogenes
entry. In contrast, chelation of host cytosolic Ca
2+
or blockade of Ca
2+
release from intracellular stores did not affect invasion. These results indicate that
L. monocytogenes
-induced mobilization of extracellular Ca
2+
by LLO and activation of downstream Ca
2+
-dependent signaling are required for efficient cell invasion.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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