Effectiveness of Disinfectants Used in Hemodialysis against both Candida orthopsilosis and C. parapsilosis Sensu Stricto Biofilms

Author:

Pires Regina Helena1,da Silva Julhiany de Fátima1,Gomes Martins Carlos Henrique2,Fusco Almeida Ana Marisa1,Pienna Soares Christiane1,Soares Mendes-Giannini Maria José1

Affiliation:

1. Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Univ Estadual Paulista—UNESP, Araraquara, São Paulo, Brazil

2. Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, São Paulo, Brazil

Abstract

ABSTRACT Biofilms have been observed in the fluid pathways of hemodialysis machines. The impacts of four biocides used for the disinfection of hemodialysis systems were tested against Candida parapsilosis sensu stricto and Candida orthopsilosis biofilms generated by isolates obtained from a hydraulic circuit that were collected in a hemodialysis unit. Acetic acid was shown to be the most effective agent against Candida biofilms. Strategies for effective disinfection procedures used for hemodialysis systems should also seek to kill and inhibit biofilms.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference36 articles.

1. Water treatment for hemodialysis: a 2005 update;Cappelli G;Contrib. Nephrol.,2005

2. Evidence of bacterial biofilm in tubing from hydraulic pathway of hemodialysis system;Man NK;Artif. Organs,1998

3. Centers for Disease Control and Prevention. 2008. Guideline for disinfection and sterilization in healthcare facilities 2008. CDC Atlanta GA. http://www.cdc.gov/hicpac/pdf/guidelines/Disinfection_Nov_2008.pdf.

4. Association for the Advancement of Medical Instrumentation (AAMI). 2006. Water treatment equipment for hemodialysis applications. ANSI/AAMI RD62, Arlington, VA.

5. Brazilian Ministry of Health. 15 June 2004 posting date. Technical regulations for the operation of dialysis services 2004. Directives no. 154/MS. Brazilian Ministry of Health Brasilia Brazil. (In Portuguese.). http://www.samu.fortaleza.ce.gov.br/legislacao/Dialise_ANVISA_RDC_154.pdf

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