Affiliation:
1. Miller Brewing Company, Milwaukee, Wisconsin 53201
Abstract
ABSTRACT
A total of 46 brewery and 15 ATCC
Pediococcus
isolates were ribotyped using a Qualicon RiboPrinter. Of these, 41 isolates were identified as
Pediococcus damnosus
using
Eco
RI digestion. Three ATCC reference strains had patterns similar to each other and matched 17 of the brewery isolates. Six other brewing isolates were similar to ATCC 25249. The other 18
P. damnosus
brewery isolates had unique patterns. Of the remaining brewing isolates, one was identified as
P. parvulus
, two were identified as
P. acidilactici
, and two were identified as unique
Pediococcus
species. The use of alternate restriction endonucleases indicated that
Pst
I and
Pvu
II could further differentiate some strains having identical
Eco
RI profiles. An acid-resistant
P. damnosus
isolate could be distinguished from non-acid-resistant varieties of the same species using
Pst
I instead of
Eco
RI. 16S rRNA gene sequence analysis was compared to riboprinting for identifying pediococci. The complete 16S rRNA gene was PCR amplified and sequenced from seven brewery isolates and three ATCC references with distinctive riboprint patterns. The 16S rRNA gene sequences from six different brewery
P. damnosus
isolates were homologous with a high degree of similarity to the GenBank reference strain but were identical to each other and one ATCC strain with the exception of 1 bp in one strain. A slime-producing, beer spoilage isolate had 16S rRNA gene sequence homology to the
P. acidilactici
reference strain, in agreement with the riboprint data. Although 16S rRNA gene sequencing correctly identified the genus and species of the test
Pediococcus
isolates, riboprinting proved to be a better method for subspecies differentiation.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
48 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献