Mutant Selection Window and Characterization of Allelic Diversity for Ciprofloxacin-Resistant Mutants of Rhodococcus equi-Reference-Cited by-同舟云学术

Mutant Selection Window and Characterization of Allelic Diversity for Ciprofloxacin-Resistant Mutants of Rhodococcus equi

Published:2010-08 Issue:8 Volume:54 Page:3520-3523
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Niwa Hidekazu¹,Lasker Brent A.²

Affiliation:

1. Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Japan

2. Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Abstract

ABSTRACT The mutant prevention concentration (MPC) for ciprofloxacin was determined for two Rhodococcus equi strains. The MPC for both strains was 32 μg/ml, which is above the peak serum concentration of ciprofloxacin obtainable by oral administration in humans. Nine single nucleotide changes corresponding to eight amino acid substitutions in the quinolone resistance-determining regions of DNA gyrase subunits A and B were characterized. Only mutants with amino acid changes in Ser-83 of GyrA were highly resistant (≥64 μg/ml). Our results suggest that ciprofloxacin monotherapy against R. equi infection may result in the emergence of ciprofloxacin-resistant mutants.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01670-09

Reference29 articles.

1. MICs of Oxazolidinones for Rhodococcus equi Strains Isolated from Humans and Animals

2. Susceptibility testing for mycobacteria nocardiae and other aerobic actinomycetes 2003

3. Cui, J., Y. Liu, R. Wang, W. Tong, K. Drlica, and X. Xhao. 2006. The mutant selection window in rabbits infected with Staphylococcus aureus. J. Infect. Dis.194:1601-1608.

4. Mutant Prevention Concentration as a Measure of Antibiotic Potency: Studies with Clinical Isolates of Mycobacterium tuberculosis

5. Drlica, K. 2003. The mutant selection window and antimicrobial resistance. J. Antimicrob. Chemother.52:11-17.

Cited by 8 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Antimicrobial Resistance Spectrum Conferred by pRErm46 of Emerging Macrolide (Multidrug)-Resistant Rhodococcus equi;Journal of Clinical Microbiology;2021-09-20

2. Ethidium bromide exposure unmasks an antibiotic efflux system in Rhodococcus equi;Journal of Antimicrobial Chemotherapy;2021-04-14

3. Antibiotic resistance genes in the Actinobacteria phylum;European Journal of Clinical Microbiology & Infectious Diseases;2019-06-27

4. Antimicrobial Resistance in Rhodococcus equi;Antimicrobial Resistance in Bacteria from Livestock and Companion Animals;2018-08-23

5. Antimicrobial Resistance in Rhodococcus equi;Microbiology Spectrum;2017-09-22