Cloning and Expression of clt Genes Encoding Milk-Clotting Proteases from Myxococcus xanthus 422-Reference-Cited by-同舟云学术

Cloning and Expression of clt Genes Encoding Milk-Clotting Proteases from Myxococcus xanthus 422

Published:2004-10 Issue:10 Volume:70 Page:6337-6341
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Poza M.¹,Prieto-Alcedo M.¹,Sieiro C.²,Villa T. G.¹

Affiliation:

1. Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela

2. Department of Microbiology, Faculty of Sciences, University of Vigo, Vigo, Spain

Abstract

ABSTRACT The screening of a gene library of the milk-clotting strain Myxococcus xanthus 422 constructed in Escherichia coli allowed the description of eight positive clones containing 26 open reading frames. Only three of them ( cltA , cltB , and cltC ) encoded proteins that exhibited intracellular milk-clotting ability in E. coli , Saccharomyces cerevisiae , and Pichia pastoris expression systems.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.10.6337-6341.2004

Reference46 articles.

1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

2. Anahit, V., A. M. Wong, T. C. Friedman, N. X. Cawley, F. E. Estivariz, H.-C. Chen, and Y. P. Loh. 1993. Purification and characterization of a paired basic residue-specific yeast aspartic protease encoded by the YAP3 gene. J. Biol. Chem.268:11968-11975.

3. Bang, M. L., I. Villadsen, and T. Sandal. 1999. Cloning and characterization of an endo-beta-1,3(4)glucanase and an aspartic proteinase from Phaffia rhodozyma CBS 6938. Appl. Microbiol. Biotechnol.51:215-222.

4. Carias, J.-R., J. Raingeaud, C. Mazaud, G. Vachon, N. Lucas, Y. Cenatiempo, and R. Julien. 1990. A chymosin-like extracellular acidic endoprotease from Myxococcus xanthus DK101. A potential tool for protein engineering. FEBS Lett.262:97-100.

5. Clarke, L., and J. Carbon. 1976. A colony bank containing synthetic Col E1 hybrid plasmids representative of the entire E. coli genome. Cell9:91-99.

Cited by 17 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Advances in research on calf rennet substitutes and their effects on cheese quality;Food Research International;2021-11

2. Understanding the biochemical characteristics of struvite bio-mineralising microorganisms and their future in nutrient recovery;Chemosphere;2020-05

3. Identification and magnetic immobilization of a pyrophilous aspartic protease from Antarctic psychrophilic fungus;Journal of Food Biochemistry;2018-10-04

4. Expression, activation and processing of a novel plant milk-clotting aspartic protease in Pichia pastoris;Journal of Biotechnology;2018-02

5. Expression of buffalo chymosin in Pichia pastoris for application in mozzarella cheese;LWT;2017-10