Affiliation:
1. Research Institute for Microbial Diseases, Osaka University, Japan.
Abstract
A mutant, Vp-MX02, having a deletion in the gene (trh) for the thermostable direct hemolysin-related hemolysin (TRH) was constructed by double-crossover gene conversion from a TRH-producing Vibrio parahaemolyticus strain, TH3996. The deleted region was the upstream half of trh. Hemolysis was completely lost, and TRH antigen was undetectable in the mutant. Administration of the mutant into ligated rabbit small intestines elicited partial, but apparent, fluid accumulation. These results suggest that the enterotoxicity of TRH-producing V. parahaemolyticus TH3996 may be attributed to the remaining C-terminal of TRH or, more likely, to an unknown virulence factor(s) in addition to TRH.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference22 articles.
1. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives;Bartimore B.;Gene,1991
2. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P1SA cryptic miniplasmid;Chang A. C. Y.;J. Bacteriol.,1978
3. Vibrio parahaemolicus infectious disease caused by Kanagawa phenomenon-negative 03:K6 originated from;Honda S.;Maldives. Jpn. J. Infect. Dis.,1987
4. Honda T. M. Abad-Lapuebla Y. Ni K. Yamamoto and T.
5. Characterization of a new thermostable direct haemolysin produced by a Kanagawa-phenomenon-negative clinical isolate of Vibrioparahaemolyticus;Miwatani;J. Gen. Microbiol.,1991
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