Modification of Sorbitol MacConkey Medium Containing Cefixime and Tellurite for Isolation of Escherichia coli O157:H7 from Radish Sprouts-Reference-Cited by-同舟云学术

Modification of Sorbitol MacConkey Medium Containing Cefixime and Tellurite for Isolation of Escherichia coli O157:H7 from Radish Sprouts

Published:2000-07 Issue:7 Volume:66 Page:3117-3118
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Fujisawa Tomohiko¹,Sata Shin¹,Aikawa Katsuhiro¹,Takahashi Takanori¹,Yamai Shiro¹,Shimada Toshio²

Affiliation:

1. Kanagawa Prefectural Public Health Laboratory, Yokohama 241-0815,1 and

2. National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640,2 Japan

Abstract

ABSTRACT A modified version of sorbitol MacConkey medium containing cefixime and tellurite (CT-SMAC medium) was produced by adding salicin and 4-methylumbelliferyl-β- d -galactopyranoside to CT-SMAC medium; this medium was designated CT-SSMAC medium and was used to isolate Escherichia coli O157:H7 from radish sprouts. Of 101 non- E. coli bacteria isolated from radish sprouts that produced colorless colonies similar to colonies of E. coli O157:H7 grown on CT-SMAC medium, 92 (91%) formed colonies that were red to pink or were β-galactosidase negative and colorless on CT-SSMAC medium. On the other hand, colonies of E. coli O157:H7 strains were colorless and β-galactosidase positive on CT-SSMAC medium. Our results suggest that CT-SSMAC medium is more selective than CT-SMAC medium for isolating E. coli O157:H7.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.7.3117-3118.2000

Reference14 articles.

1. Evaluation of methods for the isolation and detection of Escherichia coli O157 in minced beef;Bennett A. R.;Lett. Appl. Microbiol.,1995

2. Comparison of fecal microflora of elderly persons in rural and urban areas of Japan

3. The intestinal microflora of infants: composition of fecal flora in breast-fed and bottle-fed infants;Benno Y.;Microbiol. Immunol.,1984

4. Comparison of the fecal microflora in rural Japanese and urban Canadians;Benno Y.;Microbiol. Immunol.,1986

5. An improved selective medium for the isolation of Escherichia coli O157;Chapman P. A.;J. Med. Microbiol.,1991

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