Identification of Turicella otitidis isolated from a patient with otorrhea associated with surgery: differentiation from Corynebacterium afermentans and Corynebacterium auris

Author:

Renaud F N1,Grégory A1,Barreau C1,Aubel D1,Freney J1

Affiliation:

1. DERBA, UPR ES EA 1655, Laboratoire de Bactériologie, Faculté René Laennec, Lyon, France. renaud@cimac.res.univlyon1.fr

Abstract

Turicella otitidis is a newly described coryneform bacterium isolated from middle ear fluids. We report here on the diagnosis of a strain isolated from otorrhea. The API Coryne system (bioMérieux, Marcy I'Etoile, France) used alone failed to differentiate T. otitidis, Corynebacterium afermentans, and Corynebacterium auris (ANF group). Biochemical tests such as DNase, enzymatic reactions (API ZYM; bioMérieux), and carbon substrate assimilation tests (Biotype 100; bioMérieux) allow presumptive identification. However, only chemotaxonomy and molecular biology can achieve unequivocal differentiation among these three species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

1. Comparative chemotaxonomic studies of mycolic acid-free coryneform bacteria of human origin;Barreau C.;J. Clin. Microbiol.,1993

2. Clarridge J. E. and C. A. Spiegel. 1995. Corynebacterium and miscellaneous irregular gram-positive rods Erysipelothrix and Gardnerella p. 357-378. In P. R. Murray E. J. Barron M. A. Pfaller F. C. Tenover and R. H. Yolken (ed.) Manual of clinical microbiology 6th ed. ASM Press Washington D.C.

3. Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria;Freney J.;J. Clin. Microbiol.,1991

4. Heterogeneity within human-derived Centers for Disease Control and Prevention (CDC) coryneform group ANF-1-like bacteria and description of Corynebacterium auris sp. nov;Funke G.;Int. J. Syst. Bacteriol.,1995

5. A hiherto undescribed coryneform bacterium isolated from patients with otitis media;Funke G.;Med. Microbiol. Lett.,1993

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