Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria

Author:

Freney J1,Duperron M T1,Courtier C1,Hansen W1,Allard F1,Boeufgras J M1,Monget D1,Fleurette J1

Affiliation:

1. Laboratoire de Bactériologie, Faculté de Pharmacie, Lyon, France.

Abstract

A study was performed to evaluate a new manual miniaturized system, API Coryne (API-bioMérieux, Inc., La Balme les Grottes, France), in which conventional biochemical methods were used to identify 240 isolates of coryneform and related bacteria. A total of 40% of the isolates were excluded from the study because they could not be identified by conventional methods. Identifications of the 240 isolates obtained with API Coryne showed a 97.6% concordance with conventional methods (79% after 24 h of incubation, 21% after 48 h of incubation): 158 (65.8%) isolates were identified with no further testing, and extra testing was required for 76 (31.8%) isolates. In three (1.2%) cases, the organisms did not correspond to any key in the code book and could not be identified by the computer service of the manufacturer. Only three (1.2%) strains were misidentified. The system was shown to be reliable and rapid when compared with standard identification methods.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference33 articles.

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