Naturally Occurring Anti- Escherichia coli Protein Antibodies in the Sera of Healthy Humans Cause Analytical Interference in a Recombinant Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Severe Acute Respiratory Syndrome-Reference-Cited by-同舟云学术

Naturally Occurring Anti- Escherichia coli Protein Antibodies in the Sera of Healthy Humans Cause Analytical Interference in a Recombinant Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Severe Acute Respiratory Syndrome

Published:2007-01 Issue:1 Volume:14 Page:99-101
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Yip Chi Wai¹²,Hon Chung Chau¹²,Zeng Fanya¹²,Chow Ken Y. C.¹²,Chan Kwok Hung¹²,Peiris Joseph S. M.¹²,Leung Frederick C. C.¹²

Affiliation:

1. Department of Zoology

2. Department of Microbiology, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, China

Abstract

ABSTRACT We reported the analytical interference of anti- Escherichia coli protein (EP) antibodies in human sera and residual EP in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay as a possible source of false positives in severe acute respiratory syndrome serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00136-06

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