Differential Effects of Homologous Transcriptional Regulators NicR2A, NicR2B1, and NicR2B2 and Endogenous Ectopic Strong Promoters on Nicotine Metabolism in Pseudomonas sp. Strain JY-Q-Reference-Cited by-同舟云学术

Differential Effects of Homologous Transcriptional Regulators NicR2A, NicR2B1, and NicR2B2 and Endogenous Ectopic Strong Promoters on Nicotine Metabolism in Pseudomonas sp. Strain JY-Q

Published:2021-01-15 Issue:3 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Huang Chaochao¹,Shan Lihui¹,Chen Zeyu¹,He Ziliang¹,Li Jun¹,Yang Yang²,Shu Ming²,Pan Fanda²,Jiao Yang³,Zhang Fuming⁴,Linhardt Robert J.⁴,Zhong Weihong¹^ORCID

Affiliation:

1. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China

2. Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou, China

3. Technology Center, Hangzhou Liqun Environmental Protection Paper Co., Ltd., Hangzhou, China

4. Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York, USA

Abstract

This study evaluated the differential effects of homologous NicR2A and NicR2Bs and endogenous ectopic strong promoters on nicotine metabolism in Pseudomonas sp. strain JY-Q. Based on our differential analysis, a feasible strategy is presented to modify wild-type (WT) strain JY-Q by removing repressing regulatory proteins NicR2A and NicR2Bs and replacing the target promoter with strong endogenous ectopic promoters.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02457-20

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