Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Fails To Identify Nontuberculous Mycobacteria from Primary Cultures of Respiratory Samples-Reference-Cited by-同舟云学术

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Fails To Identify Nontuberculous Mycobacteria from Primary Cultures of Respiratory Samples

Published:2016-07 Issue:7 Volume:54 Page:1915-1917
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

van Eck Kim¹,Faro Dirk¹,Wattenberg Melanie¹,de Jong Arjan¹,Kuipers Saskia¹,van Ingen Jakko¹^ORCID

Affiliation:

1. Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands

Abstract

ABSTRACT We have assessed matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) identification (Bruker) of nontuberculous mycobacteria from newly positive liquid cultures of respiratory samples. Twelve (22%) of 54 isolates were identified directly from liquid medium. After subculture and with manual laser operation, this rose to 49/54 isolates (91%). MALDI-TOF MS is less promising than previously suggested.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00304-16

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