MGIT-seq for the Identification of Nontuberculous Mycobacteria and Drug Resistance: a Prospective Study-Reference-Cited by-同舟云学术

MGIT-seq for the Identification of Nontuberculous Mycobacteria and Drug Resistance: a Prospective Study

Published:2023-04-20 Issue:4 Volume:61 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fukushima Kiyoharu¹²³⁴⁵^ORCID,Matsumoto Yuki⁶,Matsuki Takanori¹,Saito Haruko⁷,Motooka Daisuke⁶⁸,Komukai Sho⁸⁹,Fukui Eriko¹⁰,Yamuchi June⁷,Nitta Tadayoshi⁷,Niitsu Takayuki²,Abe Yuko²,Nabeshima Hiroshi⁴,Nagahama Yasuharu⁴,Nii Takuro¹,Tsujino Kazuyuki¹,Miki Keisuke¹,Kitada Seigo¹¹,Kumanogoh Atsushi²⁸¹²,Akira Shizuo³⁴,Nakamura Shota⁶⁸¹²,Kida Hiroshi¹^ORCID

Affiliation:

1. Department of Respiratory Medicine, National Hospital Organization, Osaka Toneyama Medical Center, Toyonaka, Osaka, Japan

2. Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan

3. Department of Host Defense, Research Institute for Microbial Diseases (RIMD), Osaka University, Osaka, Japan

4. Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Center (WPI-IFReC), Osaka University, Osaka, Japan

5. Global Center for Medical Engineering and Informatics, Suita, Osaka, Japan

6. Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, Japan

7. Department of Clinical Laboratory, National Hospital Organization, Osaka Toneyama Medical Centre, Toyonaka, Osaka, Japan

8. Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Osaka, Japan

9. Department of Biomedical Statistics, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan

10. Department of General Thoracic surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan

11. Kitada Respiratory Clinic, Yao, Osaka, Japan

12. Center for Infectious Disease Education and Research, Japan for Infectious Disease Education and Research, Osaka University, Suita, Osaka, Japan

Abstract

Because nontuberculous mycobacterial pulmonary disease is a considerable health burden, a simple and clinically applicable analytical protocol enabling the identification of subspecies and drug-resistant disease is required to determine the treatment strategy. We aimed to develop a simplified workflow consisting only of direct sequencing of mycobacterial growth indicator tube cultures (MGIT-seq).

Funder

Japan Agency for Medical Research and Development

GlaxoSmithKline

MEXT | Japan Society for the Promotion of Science

Takeda Science Foundation

Foundation of Kinoshita Memorial Enterprise

Uehara Memorial Foundation

MSD Life Science Foundation, Public Interest Incorporated Foundation

Japan Intractable Diseases Research Foundation

Senri Life Science Foundation

Publisher

American Society for Microbiology