Affiliation:
1. Infectious Diseases Research
2. Vaccines Discovery, Wyeth, Pearl River, New York
Abstract
ABSTRACT
The presence of the tetracycline resistance determinant
tet
(M) in human clinical isolates of
Escherichia coli
is described for the first time in this report. The homologue was >99% identical to the
tet
(M) genes reported to occur in
Lactobacillus plantarum
,
Neisseria meningitidis
, and
Streptococcus agalactiae
, and 3% of the residues in its deduced amino acid sequence diverge from
tet
(M) of
Staphylococcus aureus
. Sequence analysis of the regions immediately flanking the gene revealed that sequences upstream of
tet
(M) in
E. coli
have homology to Tn
916
; however, a complete IS
26
insertion element was present immediately upstream of the promoter element. Downstream from the termination codon is an insertion sequence that was homologous to the IS
Vs1
element reported to occur in a plasmid from
Vibrio salmonicida
that has been associated with another tetracycline resistance determinant,
tet
(E). Results of mating experiments demonstrated that the
E. coli tet
(M) gene was on a mobile element so that resistance to tetracycline and minocycline could be transferred to a susceptible strain by conjugation. Expression of the cloned
tet
(M) gene, under the control of its own promoter, provided tetracycline and minocycline resistance to the
E. coli
host.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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