Affiliation:
1. Danish Institute for Food and Veterinary Research, 1790 Copenhagen V, Denmark
2. Statens Serum Institute, 2300 Copenhagen S, Denmark
Abstract
ABSTRACT
The presence of tetracycline resistance (Tc
r
) genes and class I integrons (in-1), and their ability to cotransfer were investigated in Tc
r
gram-negative (185 strains) and gram-positive (72 strains) bacteria from Danish farmland and pigsties. The isolates belonged to the groups or species
Escherichia coli
,
Enterobacter
spp.,
Arthrobacter
spp.,
Alcaligenes
spp.,
Pseudomonas
spp., and
Corynebacterium glutamicum.
The 257 isolates were screened for in-1. Eighty-one of the gram-negative isolates were also screened for the Tc
r
genes
tet
(A),
tet
(B), and
tet
(C), and all (
n
= 72) gram-positive isolates were screened for
tet
(33). Fourteen (7%) of the soil isolates and eleven (25%) of the pigsty isolates contained in-1. All isolates that contained
tet
genes also contained in-1, except one gram-negative isolate from a pigsty that contained
tet
(B). All gram-positive isolates with in-1 also contained
tet
(33). No isolates contained more than one
tet
gene. The in-1-positive isolates were tested for resistance to selected antimicrobial agents and showed resistance to three to nine drugs. Filter-mating experiments showed cotransfer of Tc
r
and class I integrons from soil isolates to
Escherichia coli
and/or
Pseudomonas putida
. We conclude that soil bacteria in close contact to manure or pigsty environment may thus have an important role in horizontal spread of resistance. Use of tetracyclines in food animal production may increase not only Tc
r
but also multidrug resistance (caused by the presence
tet
genes and in-1) in bacteria.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference33 articles.
1. Aarestrup, F. M., Y. Agersø, P. Ahrens, J. C. Jørgensen, M. Madsen, and L. B. Jensen. 2000. Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry. Vet. Microbiol.74:353-364.
2. Aarestrup, F. M., Y. Agersø, P. Gerner-Smidt, M. Madsen, and L. B. Jensen. 2000. Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark. Diagn. Microbiol. Infect.37:127-137.
3. Distribution of tetracycline resistance determinants among gram-negative bacteria isolated from polluted and unpolluted marine sediments
4. Anonymous. 2003. DANMAP 2002: use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals foods and humans in Denmark. ISSN 1600-2032. DFVF Frederiksberg Denmark.
5. Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (
aadA
) in
Enterococcus faecalis
Cited by
126 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献