Green Fluorescent Protein as a Reporter To Monitor Gene Expression and Food Colonization by Aspergillus flavus-Reference-Cited by-同舟云学术

Green Fluorescent Protein as a Reporter To Monitor Gene Expression and Food Colonization by Aspergillus flavus

Published:1999-02 Issue:2 Volume:65 Page:834-836
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Du Wanglei¹,Huang Zhengyu²,Flaherty Joseph E.¹,Wells Kevin³,Payne Gary A.¹

Affiliation:

1. Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695-76161;

2. Dow AgrosSciences LLC, Indianapolis, Indiana 46268-10542; and

3. USDA-ARS/Livestock and Poultry Science Institute, Gene Evaluation and Mapping Laboratory, Beltsville, Maryland 207053

Abstract

ABSTRACT Transformants of Aspergillus flavus containing the Aequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed green fluorescence detectable without a microscope or filters. Expression of green fluorescent protein fluorescence was correlated with resistance to aflatoxin accumulation in five corn genotypes inoculated with these transformants.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.2.834-836.1999

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