Affiliation:
1. Clinical Microbiology Institute, Tualatin, Oregon 97062.
Abstract
A multilaboratory study was undertaken to reassess the amount of calcium and magnesium that should be added to Mueller-Hinton broth when testing Pseudomonas aeruginosa against amikacin, gentamicin, isepamicin, netilmicin, and tobramycin. To achieve parity with agar dilution tests, cation-adjusted broth should contain 20 to 25 mg of calcium and 10 to 12.5 mg of magnesium per liter rather than the 50- and 25-mg/liter supplements recommended previously. For quality control of tests with contemporary media, MIC control limits should be adjusted by lowering the current MIC limits by at least 1 doubling-dilution interval.
Publisher
American Society for Microbiology
Reference9 articles.
1. Netilmicin disk susceptibility tests: effect of cations on the MIC correlates;Barry A. L.;Eur. J. Clin. Microbiol.,1987
2. Influence of cation supplements on activity of netilmicin against Pseudomonas aeruginosa in vitro and in vivo;Barry A. L.;Antimicrob. Agents Chemother.,1987
3. Cation components of Mueller-Hinton agar affecting testing of Pseudomonas aeruginosa susceptibility to gentamicin;Kenny M. A.;Antimicrob. Agents Chemother.,1990
4. National Committee for Clinical Laboratory Standards. 1986. Evaluating production lots of dehydrated Mueller-Hinton agar. Proposed standard M6-P. National Committee for Clinical Laboratory Standards Villanova Pa.
5. National Committee for Clinical Laboratory Standards. 1990. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A2 2nd ed. National Committee for Clinical Laboratory Standards Villanova Pa.
Cited by
28 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献