Analysis of bla SHV Codon 238 and 240 Allele Mixtures Using Sybr Green High-Resolution Melting Analysis-Reference-Cited by-同舟云学术

Analysis of bla SHV Codon 238 and 240 Allele Mixtures Using Sybr Green High-Resolution Melting Analysis

Published:2009-06 Issue:6 Volume:53 Page:2679-2683
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Andersson Patiyan¹,Harris Tegan¹,Tong Steven Y. C.¹,Giffard Philip M.¹

Affiliation:

1. Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia

Abstract

ABSTRACT Klebsiella pneumoniae isolates frequently contain complex mixtures of bla SHV alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum β-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00063-09

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