Repression of Phenolic Acid-Synthesizing Enzymes and Its Relation to Iron Uptake in Bacillus subtilis

Author:

Downer D. N.1,Davis W. B.1,Byers B. R.1

Affiliation:

1. Department of Microbiology, University of Mississippi School of Medicine, Jackson, Mississippi 39216

Abstract

Excretion of the metal-chelating phenolic acid, 2,3-dihydroxybenzoate, by a tryptophan-requiring strain (M-13) of Bacillus subtilis was inversely proportional to the iron added to the medium. Addition of iron as the ferric chelates of two secondary hydroxamates (ferri-schizokinen and Desferal) markedly reduced excretion. Synthesis of 2,3-dihydroxybenzoate from chorismate by extracts of B. subtilis M-13, grown in low-iron medium, was unaltered by additions of FeSO 4 , FeCl 3 , ferrischizokinen, 2,3-dihydroxybenzoate, the 2,3-dihydroxybenzoate-iron complex, or by extracts of cells grown in high-iron medium (which contained no demonstrable 2,3-dihydroxybenzoate-synthesizing activity) to the extracts of “low-iron cells.” Iron control seemed to involve repression of synthesis of the enzymes in the 2,3-dihydroxybenzoate pathway. Both ferri-schizokinen and 2,3-dihydroxybenzoate plus iron enhanced considerably the otherwise minimal repressive effects of iron at low concentrations. Ferri-schizokinen delayed derepression of the pathway in B. subtilis M-13, and reduced its rate of synthesis after derepression. Addition of FeSO 4 to derepressed cells of B. subtilis M-13 halted synthesis of the enzymes after a lag period. The effect of the ferric hydroxamates was related to the capacity of B. subtilis M-13 to incorporate 59 Fe 3+ from Desferal- 59 Fe 3+ . Cellular accumulation of 59 Fe 3+ from Desferal- 59 Fe 3+ after 20 min was nearly double that incorporated from 59 FeCl 3 .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Cited by 26 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Microbial ferric iron reductases;FEMS Microbiology Reviews;2003-06

2. Salicylic acid biosynthesis;Biochemistry and Molecular Biology of Plant Hormones;1999

3. Excretion of anthranilate and 3-hydroxyanthranilate by Saccharomyces cerevisiae: relationship to iron metabolism;Journal of General Microbiology;1992-01-01

4. Stimulation of Disease Development by Siderophores and Inhibition by Chelated Iron;Iron, Siderophores, and Plant Diseases;1986

5. Fe2+Inhibition of Extracellular Protease Synthesis inAeromonas hydrophila;Agricultural and Biological Chemistry;1985-08

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