Predicting Intraserotypic Recombination in Enterovirus 71

Author:

Woodman Andrew1,Lee Kuo-Ming2,Janissen Richard3,Gong Yu-Nong2,Dekker Nynke H.3,Shih Shin-Ru2456,Cameron Craig E.1

Affiliation:

1. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA

2. Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan

3. Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands

4. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan

5. Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan

6. Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan

Abstract

Recombination is a mechanism that contributes to genetic diversity. We describe the first assay to study EV-A71 recombination. Results from this assay mimic what is observed in nature and can be used by others to predict future recombination events within the enterovirus species A group. In addition, our results highlight the central role played by the viral RNA-dependent RNA polymerase (RdRp) in the recombination process. Further, our results show that changes to a conserved residue in the RdRp from different species groups have a similar impact on viable recombinant virus yields, which is indicative of conservation in mechanism.

Funder

HHS | National Institutes of Health

Human Frontier Science Program

Ministry of Science and Technology, Taiwan

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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