Phylogenetic Analysis of and Oligonucleotide Probe Development for Eikelboom Type 021N Filamentous Bacteria Isolated from Bulking Activated Sludge-Reference-Cited by-同舟云学术

Phylogenetic Analysis of and Oligonucleotide Probe Development for Eikelboom Type 021N Filamentous Bacteria Isolated from Bulking Activated Sludge

Published:2000-11 Issue:11 Volume:66 Page:5043-5052
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kanagawa Takahiro¹,Kamagata Yoichi¹,Aruga Shinobu¹,Kohno Tetsuro²,Horn Matthias³,Wagner Michael³

Affiliation:

1. National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Tsukuba 305-8566,1 and

2. Department of Civil and Environmental Engineering, Yamanashi University, Kofu 400-0016,2 Japan and

3. Lehrstuhl für Mikrobiologie, Technische Universität München, D-85350 Freising, Germany3

Abstract

ABSTRACT Fifteen filamentous strains, morphologically classified as Eikelboom type 021N bacteria, were isolated from bulking activated sludges. Based on comparative 16S ribosomal DNA (rDNA) sequence analysis, all strains form a monophyletic cluster together with all recognized Thiothrix species (88.3 to 98.7% 16S rDNA sequence similarity) within the gamma-subclass of Proteobacteria . The investigated Eikelboom type 021N isolates were subdivided into three distinct groups (I to III) demonstrating a previously unrecognized genetic diversity hidden behind the uniform morphology of the filaments. For in situ detection of these bacteria, 16S rRNA-targeted oligonucleotide probes specific for the entire Eikelboom type 021N- Thiothrix cluster and the Eikelboom type 021N groups I, II, and III, respectively, were designed, evaluated, and successfully applied in activated sludge.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.11.5043-5052.2000

Reference36 articles.

1. The oligonucleotide probe database

2. Combination of 16S rRNA-targeted oligonucleotide probe with flow cytometry for analyzing mixed microbial populations.;Amann R. I.;Appl. Environ. Microbiol.,1990

3. Amann R. I. In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes Molecular microbial ecology manual 3.3.6. Akkermans A. D. L. Elsas J. D. Bruij F. J. 1995 1 15 Kluwer London United Kingdom

4. Gene organization and primary structure of a ribosomal operon from Escherichia coli.;Brosius J.;J. Mol. Biol.,1981

5. The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set.;Daims H.;Syst. Appl. Microbiol.,1999

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