Role of the wbt Locus of Francisella tularensis in Lipopolysaccharide O-Antigen Biogenesis and Pathogenicity-Reference-Cited by-同舟云学术

Role of the wbt Locus of Francisella tularensis in Lipopolysaccharide O-Antigen Biogenesis and Pathogenicity

Published:2007-01 Issue:1 Volume:75 Page:536-541
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Raynaud Catherine¹²,Meibom Karin L.¹²,Lety Marie-Annick¹²,Dubail Iharilalao¹²,Candela Thomas³,Frapy Eric¹²,Charbit Alain¹²

Affiliation:

1. Université Paris Descartes, Faculté de Médecine René Descartes, Paris F-75015, France

2. Inserm, U570, Unité de Pathogénie des Infections Systémiques, Paris F-75015, France

3. Unité Toxines et Pathogénie Bactérienne CNRS-URA2172, 28 rue de Dr Roux, 75724 Paris Cedex 15, France

Abstract

ABSTRACT Francisella tularensis is a highly infectious bacterial pathogen, responsible for the zoonotic disease tularemia. We screened a bank of transposon insertion mutants of F. tularensis subsp. holarctica LVS for colony morphology alterations and selected a mutant with a transposon insertion in wbtA , the first gene of the predicted lipopolysaccharide O-antigen gene cluster. Inactivation of wbtA led to the complete loss of O antigen, conferred serum sensitivity, impaired intracellular replication, and severely attenuated virulence in the mouse model. Notably, this mutant afforded protection against a challenge against virulent LVS.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.01429-06

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