Characterization of Clonal Relatedness among the Natural Population of Staphylococcus aureus Strains by Using spa Sequence Typing and the BURP (Based upon Repeat Patterns) Algorithm

Author:

Mellmann Alexander1,Weniger Thomas2,Berssenbrügge Christoph1,Keckevoet Ursula1,Friedrich Alexander W.1,Harmsen Dag2,Grundmann Hajo3

Affiliation:

1. Institute for Hygiene

2. Department of Periodontology, University Hospital Münster, D-48149 Münster, Germany

3. Centre for Infectious Diseases Epidemiology, National Institute for Public Health and the Environment, Bilthoven 3720BA, The Netherlands

Abstract

ABSTRACT We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene ( spa ), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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