Specific Inhibition of Nitrite Oxidation by Chlorate and Its Use in Assessing Nitrification in Soils and Sediments

Author:

Belser L. W.1,Mays E. L.1

Affiliation:

1. Cawthron Institute, Nelson, New Zealand

Abstract

A method was developed to determine the ammonium oxidation rate (potential) of unenriched natural samples by measuring the nitrite produced in shaken slurries. Addition of chlorate to the samples prevented nitrite from being oxidized to nitrate. The effectiveness and specificity of chlorate were tested with pure cultures of nitrite and ammonium oxidizers, as well as in soil and sediment slurries. It was concluded that chlorate had relatively little inhibitory effect on ammonium oxidation. However, under some conditions chlorate was not completely effective in blocking nitrite oxidation, and the causes of this were investigated. The technique was designed to check for incomplete blockage.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference11 articles.

1. Nitrate reduction to nitrite, a possible source of nitrite for growth of nitrite-oxidizing bacteria;Belser L W;Appl. Environ. Microbiol.,1977

2. Diversity in the ammonia-oxidizing nitrifier population of a soil;Belser L. W.;Appl. Environ. Microbiol.,1978

3. Serological diversity within a terrestrial ammonia-oxidizing population;Belser L. W.;Appl. Environ. Microbiol.,1978

4. Bremner J. M. 1965. Nitrite by colorimetry methods p. 1219-1224 In C. A. Black (ed.) Methods of soil analysis. American Society of Agronomy Madison Wis.

5. Chittenden E. T. L. Hodgson and K. J. Dodson. 1966. Soils and agriculture of Waimea county New Zealand. N.Z. Soil Bur. Bull. no. 30.

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