Use of Erythrocytes Sensitized with Purified Pneumococcal Polysaccharides for the Assay of Antibody and Antibody-producing Cells-Reference-Cited by-同舟云学术

Use of Erythrocytes Sensitized with Purified Pneumococcal Polysaccharides for the Assay of Antibody and Antibody-producing Cells

Published:1969-03 Issue:3 Volume:17 Page:422-426
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Baker Phillip J.¹²,Stashak Philip W.¹²,Prescott Benjamin¹²

Affiliation:

1. Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

2. Laboratory of Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

Abstract

A method was described for the sensitization of erythrocytes with purified type-specific pneumococcal polysaccharide antigens using chromium chloride as a coupling agent. Erythrocytes so sensitized can be used in routine passive hemagglutination and hemolysis tests as well as in the technique of localized hemolysis-in-gel for the detection of specific antibody and specific antibody-producing cells, respectively.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.17.3.422-426.1969

Reference17 articles.

1. The significance ofmultiple antibody components in serum of immunized rabbits;Askonas B. A.;J. Immunol.,1960

2. Brevity of the inductive phase in the immune response of mice to capsular polysaccharide antigens;Baker P. J.;J. Immunol.,1967

3. A hemagglutination test and a specific depolymerase for studying immunological paralysis in mice;Brooke M.;J. Immunol.,1966

4. Breaking of immunological paralysis by injection of a specific depolymerase;Brooke M.;Nature,1966

5. Studies on the induction, specificity, prevention and breaking of immunological paralysis and immunity to pneumococcal polysaccharide;Brooke M.;J. Immunol.,1966

Cited by 42 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Molecular structures that influence the immunomodulatory properties of the lipid A and inner core region oligosaccharides of bacterial lipopolysaccharides;Infection and Immunity;1994-06

2. The glucuronoxylomannan of Cryptococcus neoformans serotype A is a type 2 T-cell-independent antigen;Infection and Immunity;1992-10

3. Structural features that influence the ability of lipid A and its analogs to abolish expression of suppressor T cell activity;Infection and Immunity;1992-07

4. Immune responses of systemic and mucosal lymphoid organs to Pnu-Imune vaccine as a function of age and the efficacy of monophosphoryl lipid A as an adjuvant;Infection and Immunity;1992-06

5. Differential sensitivity of CD8+ suppressor and cytotoxic T lymphocyte activity to bacterial monophosphoryl lipid A;Infection and Immunity;1991-09