Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli-Reference-Cited by-同舟云学术

Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

Published:2010-01-15 Issue:2 Volume:76 Page:622-626
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Andreeßen Björn¹,Lange Alvin Brian¹,Robenek Horst²,Steinbüchel Alexander¹

Affiliation:

1. Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany

2. Leibnitz-Institut für Arterioskleroseforschung, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany

Abstract

ABSTRACT We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase ( dhaB1 ) of Clostridium butyricum , propionaldehyde dehydrogenase ( pduP ) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase ( phaC1 ) of Ralstonia eutropha were expressed in recombinant Escherichia coli . Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02097-09

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