Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli-Reference-Cited by-同舟云学术

Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

Published:2010-01-15 Issue:2 Volume:76 Page:622-626
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Andreeßen Björn¹,Lange Alvin Brian¹,Robenek Horst²,Steinbüchel Alexander¹

Affiliation:

1. Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany

2. Leibnitz-Institut für Arterioskleroseforschung, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany

Abstract

ABSTRACT We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase ( dhaB1 ) of Clostridium butyricum , propionaldehyde dehydrogenase ( pduP ) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase ( phaC1 ) of Ralstonia eutropha were expressed in recombinant Escherichia coli . Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02097-09

Reference34 articles.

1. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates

2. Baba, H., N. Tanahashi, Y. Kumagai, and Y. Doi. 1992. Effects of molecular-structure on enzymatic degradation of polyesters. J. Chem. Soc. Jpn.5:527-533.

3. The Propanediol Utilization ( pdu ) Operon of Salmonella enterica Serovar Typhimurium LT2 Includes Genes Necessary for Formation of Polyhedral Organelles Involved in Coenzyme B 12 -Dependent 1,2-Propanediol Degradation

4. Brandl, H., R. A. Gross, R. W. Lenz, and R. C. Fuller. 1988. Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol.54:1977-1982.

Cited by 116 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Current Status and Challenges in the Commercial Production of Polyhydroxyalkanoate-Based Bioplastic: A Review;Processes;2024-08-15

2. Efficient bioproduction of poly(3-hydroxypropionate) homopolymer using engineered Escherichia coli strains;Bioresource Technology;2024-04

3. Cascade disassembling of spent coffee grounds into phenols, lignin and fermentable sugars en route to a green active packaging;Separation and Purification Technology;2024-04

4. Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid;Frontiers in Bioengineering and Biotechnology;2023-01-16

5. Formation of polyhydroxyalkanoates using agro and industrial waste as a substrate – a review;Biotechnology and Genetic Engineering Reviews;2023-01-14