Amphotericin B Susceptibility Testing of Yeasts with a Bactec Radiometric System

Author:

Hopfer Roy L.1,Gröschel Dieter1

Affiliation:

1. Department of Laboratory Medicine, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030

Abstract

The effect of amphotericin B on CO 2 production was studied using a Bactec 225. A radiometric procedure for yeast susceptibility testing that requires 3 h of incubation was developed. A total of 48 yeast isolates was tested in this system and the results were correlated to tube dilution studies. The drug concentration causing at least a 44% decrease in CO 2 production correlated to the tube dilution minimal inhibitory concentration in 85% of isolates tested and was within one tube dilution of the minimal inhibitory concentration in over 95% of yeasts tested.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference4 articles.

1. Rapid radiometric method of testing susceptibility of mycobacteria and slow-growing fungi to antimicrobial agents;Benitez P., G.;Antimicrob. Agents Chemother.,1974

2. Automatic radiometric measurement of antibiotic effect on bacterial growth;DeBlanc Jr., H. J., P.;Antimicrob. Agents Chemother.,1972

3. Metabolic inhibition as an index of bacterial susceptibility to drugs;DeLand F. H.;Antimicrob. Agents Chemother.,1972

4. Shadomy S. and A. Espinel-Ingroff. 1974. Susceptibility testing of antifungal agents p 569-574. In E. H. Lennette E. H. Spaulding and J. P. Truant (ed) Manual of clinical microbiology 2nd ed. American Society for Microbiology Washington D. C.

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