Affiliation:
1. Division of Nuclear Medicine, Veterans Administration Hospital and University of Florida, Gainesville, Florida 32601
Abstract
Detection of bacterial growth by measuring the release of
14
CO
2
from the metabolism of uniformly labeled
14
C-glucose has proven to be accurate, sensitive, and rapid. The inhibition of glucose metabolism by antibiotics as an index of bacterial susceptibility was evaluated based on radiometric methodology. Bacterial dose-response curves to antibiotics were defined. The susceptibility to drugs was determined within several hours after inoculation of the cultures. The pentose phosphate shunt appeared to be the major metabolic pathway involved during this period. There was a consistent relationship between the dose-response curves determined by the inhibition of glucose metabolism and the minimal inhibitory concentrations determined by a serial broth dilution technique. Inhibition of metabolism as an index of bacterial susceptibility to drugs appears to be valid, rapid, and readily applicable to available automation.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference11 articles.
1. Axelrod B. 1967. Other pathways of carbohydrate metabolism. In David M. Greenberg (ed.) Metabolic pathways vol. I. Academic Press Inc. New York.
2. Effect of varying preincubation and diffusion times on antibiotic disc susceptibility testing;Bourgeois L. D.;Antimicrob. Ag. Chemother.,1966
3. Burrows W. and J. W. Moulder. 1968. Immunity p. 340. In William Burrows (ed.) Textbook of microbiology: the biology of microorganisms. W.B. Saunders Co. Philadelphia.
4. Automated radiometric detection of bacteria in 2,967 blood cultures;DeBlanc H. J.;Appl. Microbiol.,1971
5. Early detection of bacterial growth with carbon-14 labelled glucose;DeLand F. H.;Radiology,1969
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