A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences-Reference-Cited by-同舟云学术

A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences

Published:1997-01 Issue:1 Volume:63 Page:310-313
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Blais B W¹,Turner G¹,Sooknanan R¹,Malek L T¹

Affiliation:

1. Laboratory Services Division, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.

Abstract

A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (< 10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.63.1.310-313.1997

Reference13 articles.

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2. Transcriptional enhancement of the Listeria monocytogenes PCR and simple immunoenzymatic assay of the product using anti-RNA:DNA antibodies;Blais B. W.;Appl. Environ. Microbiol.,1994

3. Applicability of the PCR technique in the food testing laboratory: identification of Listeria monocytogenes;Blais B. W.;Biotechnol. Tech.,1995

4. A nucleic acid sequence-based amplification (NASBA) system for Listeria monocytogenes and simple method for detection of amplimers;Blais B. W.;Biotechnol. Tech.,1996

5. Nucleic acid sequence-based amplification;Compton J.;Nature (London),1991

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