Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2 332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus-Reference-Cited by-同舟云学术

Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2 332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus

Published:2016-02 Issue:2 Volume:54 Page:439-442
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chen Xiaowei¹,Song Cailing¹,Liu Yun¹,Qu Liandong¹,Liu Dafei¹,Zhang Yun¹,Liu Ming¹

Affiliation:

1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China

Abstract

ABSTRACT For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2 332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2 332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2 332-452 ELISA may be a preferable method for detecting antibodies against AMDV.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02625-15

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