Influence of Coliform Source on Evaluation of Membrane Filters

Author:

Brodsky M. H.1,Schiemann D. A.1

Affiliation:

1. Ministry of Health, Laboratory Services Branch, Toronto, Ontario MSW 1R5

Abstract

Four brands of membrane filters were examined for total and fecal coliform recovery performance by two experimental approaches. Using diluted EC broth cultures of water samples, Johns-Manville filters were superior to Sartorius filters for fecal coliform but equivalent for total coliform recovery. Using river water samples, Johns-Manville filters were superior to Sartorius filters for total coliform but equivalent for fecal coliform recovery. No differences were observed between Johns-Manville and Millipore or Millipore and Sartorius filters for total or fecal coliform recoveries using either approach, nor was any difference observed between Millipore and Gelman filters for fecal coliform recovery from river water samples. These results indicate that the source of the coliform bacteria has an important influence on the conclusions of membrane filter evaluation studies.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference7 articles.

1. American Public Health Association. 1971. Standard methods for the examination of water and wastewater 13th ed. American Public Health Association Inc. New York.

2. Comparison of autoclave and ethylene oxide-sterilized membrane filters used in water quality studies;Dutka B. J.;Appl. Microbiol.,1974

3. Guenther W. C. 1964. Analysis of variance. Prentice-Hall Inc. Englewood Cliffs N.J.

4. Harris F. L. 1974. Coliform recoveries on membrane filters p. 4. In J. B. Anderson (ed.) Analytical quality control laboratory newsletter July. -U.S. Environmental Protection Agency Washington D.C.

5. Evaluating the membrane fecal coliform test by using Escherichia coli as the indicator organism;Hufham J. B.;Appl. Microbiol.,1974

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