Affiliation:
1. Life Science Laboratory, University of Missouri, Rolla, Missouri 65401
Abstract
The fecal coliform membrane filter method (MFC) currently used in water pollution analysis was evaluated by using two strains of
Escherichia coli
, a known fecal coliform, as the indicator organism. A large relative error in the results obtained with this method was found to be dependent upon the brand of membrane filter employed, the medium, and the temperature of incubation. MFC densities varied between 10 and 60% of the densities determined by means of total bacteria counts and total coliform counts performed at 35 C. Due to the large relative error encountered, the MFC method cannot be recommended as an analytical tool for the laboratory enumeration of
E. coli
. The results do show that the MFC method can be used at 35 C for enumeration of
E. coli
and for differential counts of
E. coli
and
Enterobacter aerogenes
.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference4 articles.
1. American Public Health Association. 1971. Standard methods for the examination of water and wastewater 13th ed. American Public Health Association Inc. New York.
2. Fecal coliform organism medium for the membrane filter technique;Geldreich E. E.;J. Amer. Water Works Ass.,1965
3. McKee J. E. and H. W. Wolf. 1963. Water quality criteria 2nd ed. California State Water Resources Control Board publ. no. 3-A Sacramento.
4. Comparison of Gelman and Millipore membrane filters for enumerating fecal coliform bacteria;Presswood W. G.;Appl. Microbiol.,1973
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