Uncharacterized Bacterial Structures Revealed by Electron Cryotomography

Author:

Dobro Megan J.1,Oikonomou Catherine M.2,Piper Aidan1,Cohen John1,Guo Kylie2,Jensen Taylor2,Tadayon Jahan2,Donermeyer Joseph2,Park Yeram2,Solis Benjamin A.3,Kjær Andreas4,Jewett Andrew I.2,McDowall Alasdair W.2,Chen Songye2,Chang Yi-Wei2,Shi Jian5,Subramanian Poorna2,Iancu Cristina V.6,Li Zhuo7,Briegel Ariane8,Tocheva Elitza I.9,Pilhofer Martin10,Jensen Grant J.211ORCID

Affiliation:

1. Hampshire College, Amherst, Massachusetts, USA

2. California Institute of Technology, Pasadena, California, USA

3. University at Albany, SUNY, Albany, New York, USA

4. University of Southern Denmark, Odense, Denmark

5. National University of Singapore, Singapore, Republic of Singapore

6. Rosalind Franklin University of Medicine and Science, Chicago, Illinois, USA

7. City of Hope, Duarte, California, USA

8. Leiden University, Sylvius Laboratories, Leiden, Netherlands

9. University of Montreal, Montreal, Quebec, Canada

10. ETH Zurich, Zurich, Switzerland

11. Howard Hughes Medical Institute, Pasadena, California, USA

Abstract

ABSTRACT Electron cryotomography (ECT) can reveal the native structure and arrangement of macromolecular complexes inside intact cells. This technique has greatly advanced our understanding of the ultrastructure of bacterial cells. We now view bacteria as structurally complex assemblies of macromolecular machines rather than as undifferentiated bags of enzymes. To date, our group has applied ECT to nearly 90 different bacterial species, collecting more than 15,000 cryotomograms. In addition to known structures, we have observed, to our knowledge, several uncharacterized features in these tomograms. Some are completely novel structures; others expand the features or species range of known structure types. Here, we present a survey of these uncharacterized bacterial structures in the hopes of accelerating their identification and study, and furthering our understanding of the structural complexity of bacterial cells. IMPORTANCE Bacteria are more structurally complex than is commonly appreciated. Here we present a survey of previously uncharacterized structures that we observed in bacterial cells by electron cryotomography, structures that will initiate new lines of research investigating their identities and roles.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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