Efficiently Editing the Vaccinia Virus Genome by Using the CRISPR-Cas9 System-Reference-Cited by-同舟云学术

Efficiently Editing the Vaccinia Virus Genome by Using the CRISPR-Cas9 System

Published:2015-03-04 Issue:9 Volume:89 Page:5176-5179
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J. Virol.

Author:

Yuan Ming,Zhang Wensheng,Wang Jun,Al Yaghchi Chadwan,Ahmed Jahangir,Chard Louisa,Lemoine Nick R.,Wang Yaohe

Abstract

Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference27 articles.

1. Safety of and immunological response to a recombinant vaccinia virus vaccine expressing HIV envelope glycoprotein;Lancet,1991

2. A national multicenter phase 2 study of prostate-specific antigen (PSA) pox virus vaccine with sequential androgen ablation therapy in patients with PSA progression: ECOG 9802;Eur Urol,2014

3. Vaccinia as a vector for gene delivery;Expert Opin Biol Ther,2004

4. Reflections on the early development of poxvirus vectors;Vaccine,2013

5. Intravenous delivery of a multi-mechanistic cancer-targeted oncolytic poxvirus in humans;Nature,2011

Cited by 92 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A virus-like particle candidate vaccine based on CRISPR/Cas9 gene editing technology elicits broad-spectrum protection against SARS-CoV-2;Antiviral Research;2024-05

2. Protection of Chickens against H9N2 Avian Influenza Isolates with a Live Vector Vaccine Expressing Influenza Hemagglutinin Gene Derived from Y280 Avian Influenza Virus;Animals;2024-03-12

3. Can the Revolution in mRNA-Based Vaccine Technologies Solve the Intractable Health Issues of Current Ruminant Production Systems?;Vaccines;2024-01-31

4. Oncolytic vaccinia virus and cancer immunotherapy;Frontiers in Immunology;2024-01-12

5. The Viral Protein Poly(A) Polymerase Catalytic Subunit Interacts with Guanylate-Binding Proteins 2 to Antagonize the Antiviral Ability of Targeting Ectromelia Virus;International Journal of Molecular Sciences;2023-10-30