The SARS-CoV nsp12 Polymerase Active Site Is Tuned for Large-Genome Replication-Reference-Cited by-同舟云学术

The SARS-CoV nsp12 Polymerase Active Site Is Tuned for Large-Genome Replication

Published:2022-08-24 Issue:16 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Campagnola Grace¹,Govindarajan Vishnu¹,Pelletier Annelise¹,Canard Bruno²,Peersen Olve B.¹^ORCID

Affiliation:

1. Department of Biochemistry & Molecular Biology, Colorado State University, Fort Collins, Colorado, USA

2. Centre National de la Recherche Scientifique, Aix-Marseille Université CNRS UMR 7257, AFMB, Marseille, France

Abstract

Replicating large genomes represents a challenge for RNA viruses because fast RNA synthesis is needed to escape innate immunity defenses, but faster polymerases are inherently low-fidelity enzymes. Nonetheless, the coronaviruses replicate their ≈30-kb genomes using the core polymerase structure and mechanism common to all positive-strand RNA viruses.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Innovative Medicines Initiative

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.00671-22

Reference61 articles.

1. One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities

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