Optimized Recombinant Expression and Purification of the SARS‐CoV‐2 Polymerase Complex

Abstract

AbstractAn optimized protocol has been developed to express and purify the core RNA‐dependent RNA polymerase (RdRP) complex from the severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2). The expression and purification of active core SARS‐CoV‐2 RdRp complex is challenging due to the complex multidomain fold of nsp12, and the assembly of the multimeric complex involving nsp7, nsp8, and nsp12. Our approach adapts a previously published method to express the core SARS‐CoV‐2 RdRP complex in Escherichia coli and combines it with a procedure to express the nsp12 fusion with maltose‐binding protein in insect cells to promote the efficient assembly and purification of an enzymatically active core polymerase complex. The resulting method provides a reliable platform to produce milligram amounts of the polymerase complex with the expected 1:2:1 stoichiometry for nsp7, nsp8, and nsp12, respectively, following the removal of all affinity tags. This approach addresses some of the limitations of previously reported methods to provide a reliable source of the active polymerase complex for structure, function, and inhibition studies of the SARS‐CoV‐2 RdRP complex using recombinant plasmid constructs that have been deposited in the widely accessible Addgene repository. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Expression and production of SARS‐CoV‐2 nsp7, nsp8, and nsp12 in E. coli cellsSupport Protocol: Establishment and maintenance of insect cell culturesBasic Protocol 2: Generation of recombinant baculovirus in Sf9 cells and production of nsp12 fusion protein in T. ni cellsBasic Protocol 3: Purification of the SARS‐CoV‐2 core polymerase complex