Differential Analysis of Sulfhydryl and Disulfide Groups of Intact Spores

Abstract

Blankenship , L. C. (Eastern Utilization Research and Development Division, Washington D.C.), and M. J. Pallansch . Differential analysis of sulfhydryl and disulfide groups of intact spores. J. Bacteriol. 92: 1615–1617. 1966.—Fluorescence quenching of fluorescein mercuric acetate in alkaline medium (1 n NaOH) was found to be an accurate, sensitive method for differential analysis of sulfhydryl and disulfide groups of intact bacterial spores. Disulfide content of three species of spores was found to be slightly higher than reported by other workers. A significant increase in sulfhydryl group concentration was observed after physiological germination, indicating participation of a disulfide-reducing system in the germination process.