Rapid Quantitative Method for Salmonella Detection in Polluted Waters-Reference-Cited by-同舟云学术

Rapid Quantitative Method for Salmonella Detection in Polluted Waters

Published:1971-04 Issue:4 Volume:21 Page:662-667
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Cheng Chu Ming¹,Boyle William C.¹,Goepfert J. M.¹

Affiliation:

1. Department of Civil Engineering and Food Research Institute, Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

Abstract

A procedure has been developed for the enumeration of salmonellae in polluted waters using several modifications of existing techniques. Confirmation of salmonellae is achieved within 48 hr. This procedure includes selective enrichment in m-Tetrathionate Broth (22 ± 1 hr), plating on Brilliant Green Sulfa Agar (20 ± 1 hr), and confirmation by flagellar (H) agglutination of the growth in a mannosecontaining medium (6 ± 1 hr). An incubation temperature of 41.5 C was used throughout this procedure. Dilution to extinction techniques (most probable number) were employed to enumerate salmonellae. Large sample volumes were concentrated through the use of membrane filters. This technique proved to be rapid and reliable for the enumeration of salmonellae in water, waste water, and waste-water sludges.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.21.4.662-667.1971

Reference13 articles.

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5. Fecal coliform organism medium for the membrane filter technique;Geldreich E. E.;J. Amer. Water Works Ass.,1965

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