Affiliation:
1. Institute of Life Sciences, Ajinomoto Co., Inc., Kawasaki 210-8681, Japan
Abstract
ABSTRACT
We previously observed secretion of active-form transglutaminase in
Corynebacterium glutamicum
by coexpressing the subtilisin-like protease SAM-P45 from
Streptomyces albogriseolus
to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native
Streptoverticillium mobaraense
enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference18 articles.
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4. Characterization of a second lysine decarboxylase isolated from Escherichia coli
5. Secretion of Active-Form
Streptoverticillium mobaraense
Transglutaminase by
Corynebacterium glutamicum
: Processing of the Pro-Transglutaminase by a Cosecreted Subtilisin-Like Protease from
Streptomyces albogriseolus
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