Affiliation:
1. Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706
Abstract
ABSTRACT
Pantothenate is the product of the ATP-dependent condensation of pantoate and β-alanine and is a direct precursor of coenzyme A. A connection exists between pantothenate biosynthesis and thiamine biosynthesis in
Salmonella enterica
serovar Typhimurium since derivatives of a
purF
mutant that can grow (on glucose medium) in the absence of thiamine excrete pantothenate. We show here that the causative mutation in three such mutants was the addition of a CG base pair upstream of the
panB
gene. This base addition brings the spacing between the −10 and −35 hexamers of the promoter to a consensus spacing of 17 bp and results in increased transcription of the
pan
operon. Furthermore, overexpression of PanB caused by this mutation, or by other means, was necessary and sufficient to increase pantothenate production and allow PurF-independent thiamine synthesis on glucose medium.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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