Luciferase Detection during Stationary Phase in Lactococcus lactis-Reference-Cited by-同舟云学术

Luciferase Detection during Stationary Phase in Lactococcus lactis

Published:2007-07-15 Issue:14 Volume:73 Page:4704-4706
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bachmann Herwig¹,Santos Filipe¹²,Kleerebezem Michiel¹²,van Hylckama Vlieg Johan E. T.¹²

Affiliation:

1. NIZO food research, Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 20, 6710 BA Ede, The Netherlands

2. TI Food & Nutrition, P.O. Box 557,6700 AN Wageningen, The Netherlands

Abstract

ABSTRACT The luminescence signal of luxAB -encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis , detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02807-06

Reference19 articles.

1. Characterization of In Vivo Reporter Systems for Gene Expression and Biosensor Applications Based on luxAB Luciferase Genes

2. Identification of Lactobacillus plantarum Genes That Are Induced in the Gastrointestinal Tract of Mice

3. Riboflavin Production in Lactococcus lactis : Potential for In Situ Production of Vitamin-Enriched Foods

4. The Riboflavin Transporter RibU in Lactococcus lactis : Molecular Characterization of Gene Expression and the Transport Mechanism

5. Regulation of Expression of the Lactococcus lactis Histidine Operon

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